| 土壤来源的五个苏云金芽孢杆菌新亚种的鉴定 |
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| 引用本文： | 李荣森,高梅影,戴顺英,李小刚.土壤来源的五个苏云金芽孢杆菌新亚种的鉴定[J].微生物学报,1999,39(2):154-159. |
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| 作者姓名： | 李荣森 高梅影 戴顺英 李小刚 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉 430071 |
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| 摘 要： | 从中国土壤中分离的大量苏云金芽孢杆菌菌株中鉴定出H42、H43、H56、H60及H62等5种新H血清型，并进行了形态、培养特征、生化反应、晶体蛋白质成分及毒力特性等项检测鉴定，鉴定了5个苏云金芽孢杆菌新亚种：
Bacillus thuringiensis subsp. jinghongiensis (H42), B.thuringiensis subsp. guiyangiensis (H43),B.thuringiensis subsp. rongseni(H56),B.thuringiensis subsp. pingluonsis(H60)及B.thuringiensis subsp. zhaodongensis(H62)
。毒力生物测定证明5个新亚种的代表菌株对棉铃虫(Heliothis armigera)\,小菜蛾(Plutella xylostella)\,柳蓝叶甲(Plagiodera versicolora)幼虫均无毒力。H42、H43、H56、H60对埃及伊蚊(Aedes aegypti)\,斑须按蚊(Anopheles stephensi)及尖音库蚊(Culex pipiens)亦均无毒；H62对埃及伊蚊无毒，但对尖音库蚊与斑须按蚊有低毒。
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| 关 键 词： | 苏云金芽孢杆菌，新亚种，鉴定 |
THE IDENTIFICATION OF 5 NEW SEROTYPES OF BACILLUS THURINGIENSIS FROM SOIL IN CHINA |
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| Li Rongsen\ Gao Meiying\ Dai Shunying\ Li Xiaogang.THE IDENTIFICATION OF 5 NEW SEROTYPES OF BACILLUS THURINGIENSIS FROM SOIL IN CHINA[J].Acta Microbiologica Sinica,1999,39(2):154-159. |
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| Authors: | Li Rongsen\ Gao Meiying\ Dai Shunying\ Li Xiaogang |
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| Institution: | Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071. |
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| Abstract: | Five new strains of Bacillus thuringiensis (Bt) from soil in China were classified. 5 new H serotypes:H42,H43,H56,H60 and H62 were identified. There were some distinction in the morphology, biochemical reaction and toxicity against species of insect. Five new Bt subspecies were identified as follows: Strain YGd22 03 Bacillus thuringiensis subsp. jinghongiensis, H42;Strain KK31 01 Bacillus thuringiensis subsp. guiyangiensis ,H43;Strain SCg04 02 B.thuringiensis subsp. rongseni ,H56;Strain NXP15 04 B.thuringiensis subsp. pingluonsis ,H60;Strain HZ39 04 B.thuringiensis subsp. zhaodongensis ,H62. |
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| Keywords: | Bacillus thuringiensis New subspeciens Identification |
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