猪瘟病毒在PK细胞和MPK细胞中繁殖过程的研究

以猪瘟病毒疫苗Ｔｈｉｖｅｒｖａｌ株（Ｔ株）为实验材料，研究该病毒株在ＰＫ１５细胞中增殖的基本特性与规律。在ＰＫ１５细胞中，猪瘟病毒Ｔ株在感染后１２ｈ即可检测到子代病毒粒子。接毒后４８ｈ，几乎所有的细胞都被病毒感染；到６０ｈ，释放到培养液中有活性的病毒粒子达到最高峰，为１０７ＴＣＩＤ５０／ｍＬ。培养液中的病毒粒子在３７℃半寿期只有３个小时。同时，建立了ＭＰＫ细胞ＣＳＦＶＴ株的感染模式，其ＣＳＦＶ的滴度可达１０８ＴＣＩＤ５０／ｍＬ。在此基础上，用抗ＣＳＦＶ包膜蛋白Ｅ２和非结构蛋白ｐ１２０的单克隆抗体显示了病毒在细胞中增殖的部位，进而应用电镜技术观察到成熟的病毒粒子及可能处在不同发育阶段的子代病毒粒子

The morphological structure of classical swine fever virus and some characteristics of its multiplication]

Some characteristics of the multiplication of classical swine fever virus(CSFV) Thiverval strain were studied by means of the immunofluorescence technique. Under optimum culture conditions, the concentration of CSFV in the culture liquid multiplicated by MPK cells is ten times higher than by PK-15 cells. The half-life period of CSFV at 37 degrees C is 3 hour. The location of CSFV's replication in host cells is detected by the monoclonal antibodies of the structural protein E2 and the non-structural protein p120 of CSFV. Based on the above results, the ultrastructure of CSFV particles inside host cells was further studied using electron microscope and the changes of the ultrastructure of CSFV-infected cells are shown. The possible reasons for lower viral titre are also discussed in this paper.