| 酵母菌属间原生质体融合构建高温酵母菌株 |
| |
| 引用本文： | 文铁桥,赵学慧.酵母菌属间原生质体融合构建高温酵母菌株[J].微生物学报,1999,39(2):141-147. |
| |
| 作者姓名： | 文铁桥 赵学慧 |
| |
| 作者单位： | 华中农业大学食品科技系 武汉 430070 |
| |
| 摘 要： | 酿酒酵母(Saccharomyces cerevisiae) A001和克鲁维酵母(Kluyveromyces sp.) Y034属间原生质体融合构建高温酵母菌株。对制备高再生活性原生质体及融合子细胞形态、生理化特征、同工酶性质、遗传稳定性和高温发酵等方面进行了研究。结果表明，融合子AY023和AY680遗传性能稳定，表达了双亲优良性状，获得了在45℃培养条件下产酒率7.4%的属间融合菌株，是目前已见文献报道的产酒率最高的高温(45℃)酵母菌株。
|
| 关 键 词： | 酿酒酵母， 克鲁维酵母， 原生质体融合， 高温酒精发酵 |
CONSTRUCTION OF THERMOTOLERANT ETHANOL PRODUCING YEAST BY PROTOPLAST FUSION |
| |
| Wen Tieqiao\ Zhao Xuehui.CONSTRUCTION OF THERMOTOLERANT ETHANOL PRODUCING YEAST BY PROTOPLAST FUSION[J].Acta Microbiologica Sinica,1999,39(2):141-147. |
| |
| Authors: | Wen Tieqiao\ Zhao Xuehui |
| |
| Institution: | Department of Food and Technology, Huazhong Agricultural University, Wuhan 430070. |
| |
| Abstract: | Protoplast fusion between Saccharomyces cerevisiae A001 and Kluyveromyces Y034 has been studied. A001 is a high alcohol yield yeast and can ferment maltose into ethanol, but can not grow at 45 degrees C. However, Y034 can grow above 45 degrees C but has a lower alcohol yield and can not ferment maltose. We obtained the fusants which were tested in several aspects such as cell morphology, physiological and biochemical feature, isozyme patterns analysis, alcohol fermentation at high temperature(45 degrees C) and so on. The fusant AY023 with the final ethanol concentration of 7.4% has been selected, which is thermotolerant yeast with the highest alcohol yield at 45 degrees C until now. |
| |
| Keywords: | Saccharomyces cerevisiae Kluyveromyces Protoplast fusion High temperature fermentation |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
