| 原核表达的天花粉蛋白和另外两种蛋白具有体外抗真菌活性 |
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| 引用本文： | 胡苹,安成才,李毅,陈章良.原核表达的天花粉蛋白和另外两种蛋白具有体外抗真菌活性[J].微生物学报,1999,39(3):234-240. |
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| 作者姓名： | 胡苹 安成才 李毅 陈章良 |
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| 作者单位： | 北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室 |
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| 基金项目： | 国家科委医药创新博士项目 |
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| 摘 要： | 将天花粉蛋白、烟草几丁质酶和烟草β１，３葡聚糖酶的结构基因分别克隆到原核表达系统中进行表达，对三种基因的原核表达产物的粗提取物分别进行体外抗菌活性检测，发现三种蛋白质均有抗真菌活性。三种蛋白中任意两种蛋白的组合，其抗真菌活性显著高于单一组分的抗真菌活性。三种蛋白共同作用时，获得了更好的抗真菌效果
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| 关 键 词： | 天花粉蛋白 烟草几丁质酶 烟草β1 3葡聚糖酶 真菌病害 |
Prokaryotic expressed trichosanthin and other two proteins have anti-fungal activity in vitro] |
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| P Hu,C An,Y Li,Z Chen.Prokaryotic expressed trichosanthin and other two proteins have anti-fungal activity in vitro][J].Acta Microbiologica Sinica,1999,39(3):234-240. |
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| Authors: | P Hu C An Y Li Z Chen |
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| Institution: | National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871. |
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| Abstract: | DNAs encoding Trichosanthin, tobacco class I Chitinase and tobacco class I beta-1, 3-Glucanase were expressed in E. coli, respectively. The expression products were assayed for their anti-fungal activity. All of three kinds of proteins show anti-fungal activity. When two of them combined, this activity was enhanced greatly. When three of them combined, the stronger anti-fungal activity was observed. |
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