Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.) |
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| Authors: | P. I. P. Perera V. R. M. Vidhanaarachchi T. R. Gunathilake D. M. D. Yakandawala V. Hocher J. L. Verdeil L. K. Weerakoon |
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| Affiliation: | (1) Tissue Culture Division, Coconut Research Institute, Lunuwila, 61150, Sri Lanka;(2) Department of Botany, University of Peradeniya, Peradeniya, Sri Lanka;(3) Institute for Research and Development (IRD), UMR 1098 BEPC, IRD, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 1, France;(4) CIRAD, TA40/02 Avenue Agropolis, 34398 Montpellier Cedex 5, France;(5) Postgraduate Institute of Science, University of Peradeniya, Peradeniya, Sri Lanka |
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| Abstract: | Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 μM thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 μM 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA3) to conversion medium containing 5 μM 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries. |
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