意大利蜜蜂胚后发育过程中中肠上皮组织细胞的更替

中肠是昆虫消化、 吸收营养物质的主要部位。本研究通过形态解剖、 BrdU免疫组织化学和原位末端转移酶标记(TUNEL)细胞凋亡检测等技术， 对意大利蜜蜂Apis mellifera ligustica中肠胚后发育过程中细胞的增殖和凋亡模式进行了比较研究。结果表明：意大利蜜蜂幼虫发育早期， 中肠的增加主要来自于上皮细胞的分裂以及再生细胞的增殖。在变态发育期间， 中肠上皮经历了广泛的重组， 由再生细胞重新形成的蛹上皮替代了幼虫上皮。再生细胞在蜜蜂中肠的整个发育阶段始终存在， 为中肠的生长和更替提供了主要的细胞来源。本研究为昆虫组织细胞自噬和凋亡机制的研究提供一定的证据。

Replacement of midgut epithelium in Apis mellifera ligustica (Hymenoptera: Apidae) during postembryonic development

The midgut epithelium of insects is responsible for enzyme production, digestion, and secretion. The proliferation and apoptosis pattern of midgut in Apis mellifera ligustica was comparatively studied by using 5-bromo-2-deoxyuridine (BrdU) incorporation and the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique. The results showed that during larval development the midgut size increase resulted from digestive cell proliferation and from the increase of digestive cell num...