Mechanisms of Adhesion Among Cells of the Early Chick Blastoderm

Cells from the extraembryonic endoderm of the gastrulating chick embryo contain a β-d-galactoside-binding lectin inhibited by thiodigalactoside (TDG). TDG inhibits the aggregation of freshly prepared cells. In these fresh cell suspensions, adhesion is also inhibited when purified lectin is added to the aggregation assay. If these cells are incubated at 22° C their adhesion decreases. Associated with this is an increase in lectin activity in the cell supernatants. In these incubated cells aggregation is stimulated by TDG and desialyzed fetuin. These data suggest that the lectin may have a role to play in cellular adhesion. Under some experimental conditions extraembryonic endoderm cells from rosettes with trypsinized glutaraldehyde-fixed rabbit erythrocytes. This phenomenon is inhibited, to a certain extent, by TDG.