Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica |
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Authors: | Mitra Amiri-Jami Haifeng Wang Yukio Kakuda Mansel W Griffiths |
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Institution: | (1) Department of Food Science, University of Guelph, N1G 2W1 Guelph, ON, Canada;(2) Canadian Research Institute for Food Safety, University of Guelph, N1G 2W1 Guelph, ON, Canada |
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Abstract: | Transposon Tn5 mutagenesis was used to generate random mutations in Shewanella baltica MAC1, a polyunsaturated fatty acid (PUFA)-producing bacterium. Three mutants produced 3–5 times more eicosapentaenoic acid (EPA 20:5 n−3) compared to the wild type at 10°C. One of the mutants produced 0.3 mg EPA g−1 when grown at high temperature (30°C). Moreover, 2 mg docosahexaenoic acid (DHA 22:6 n−3) g−1 was produced by S. baltica mutants at 4°C. Sequencing of insertion mutation(s) showed 96% homology to trimethylamine N-oxide (TMAO) reductase gene and 85% homology to rRNA operons of E. coli. Tn5 transposon mutagenesis therefore is a suitable technique to increase PUFA formation in bacteria. |
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Keywords: | Docosahexaenoic acid Eicosapentaenoic acid Shewanella Transposon mutagenesis |
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