Expression of Embryonic Haemoglobin in ts AEV-Transformed Embryonic Erythroid Cells During Temperature-Induced Differentiation |
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Authors: | CHRISTA FREUDENSTEIN HARTMUT BEUG STEVEN PALMIERI THOMAS GRAF |
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Affiliation: | Institut für Virusforschung, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900 Heidelberg, Federal Republic of Germany |
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Abstract: | Cells prepared from 1-day-old chick blastoderms were infected with a temperature-sensitive mutant of avian erythroblastosis virus ( ts AEV). Clonal strains of transformed erythroblasts were isolated from the infected blastoderm cells. By shift to the nonpermissive temperature, these cells could be induced to differentiate into erythrocyte-like cells which expressed embryonic haemoglobins. Embryonic haemoglobins could not be detected in ts AEV-transformed erythroblasts from adult bone marrow when induced to differentiate under the same conditions. In contrast to normal primitive erythrocytes, ts AEV-infected embryonic erythroblasts differentiated in vitro expressed also adult haemoglobin. These results suggest an influence of the haematopoietic environment on the switch from embryonic to adult erythrocytes. |
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