Construction and validation of the APOCHIP,a spotted oligo-microarray for the study of beta-cell apoptosis |
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| Authors: | Nils?E?Magnusson mailto:nm@ki.au.dk" title=" nm@ki.au.dk" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Alessandra?K?Cardozo,Mogens?Kruh?ffer,Decio?L?Eizirik,Torben?F??rntoft,Jens?L?Jensen |
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| Affiliation: | 1.Molecular Diagnostic Laboratory, Department of Clinical Biochemistry,Aarhus University Hospital,Denmark;2.Department of Theoretical Statistics, Department of Mathematical Sciences,Aarhus University,Denmark;3.Laboratory of Experimental Medicine,Université Libre de Bruxelles,Brussels,Belgium |
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| Abstract: | ![]()
Background Type 1 diabetes mellitus (T1DM) is a autoimmune disease caused by a long-term negative balance between immune-mediated beta-cell damage and beta-cell repair/regeneration. Following immune-mediated damage the beta-cell fate depends on several genes up- or down-regulated in parallel and/or sequentially. Based on the information obtained by the analysis of several microarray experiments of beta-cells exposed to pro-apoptotic conditions (e.g. double stranded RNA (dsRNA) and cytokines), we have developed a spotted rat oligonucleotide microarray, the APOCHIP, containing 60-mer probes for 574 genes selected for the study of beta-cell apoptosis. |
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