An algorithm for automatic evaluation of the spot quality in two-color DNA microarray experiments |
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Authors: | Email author" target="_blank">Eugene?NovikovEmail author Emmanuel?Barillot |
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Institution: | (1) Service Bioinformatique, Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France |
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Abstract: | Background Although DNA microarray technologies are very powerful for the simultaneous quantitative characterization of thousands of
genes, the quality of the obtained experimental data is often far from ideal. The measured microarrays images represent a
regular collection of spots, and the intensity of light at each spot is proportional to the DNA copy number or to the expression
level of the gene whose DNA clone is spotted. Spot quality control is an essential part of microarray image analysis, which
must be carried out at the level of individual spot identification. The problem is difficult to formalize due to the diversity
of instrumental and biological factors that can influence the result. |
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