双歧醋对高脂饮食大鼠血脂影响的机制探讨

目的 探讨双歧醋对高脂饮食大鼠血脂的影响机制.方法 48只SD大鼠被随机分成双歧醋低剂量组1.8 mL/(kg·BW)]、中剂量组3.4 mL/(kg· BW)]、高剂量组6.8 mL/(kg·BW)]和市售醋组3.4 mL/(kg·BW)]、高脂模型组以及基础对照组,观察双歧醋对高脂饮食大鼠血脂的影响作用机制,测定指标包括大鼠肝脏脂质、大鼠LDLR的免疫组化分析,HMGCoA还原酶和LDLR的RT-PCR分析.结果 双歧醋各组对于HMGCoA还原酶表达有抑制作用,但可以上调LDLR的基因和蛋白质表达.结论 双歧醋可以通过调节HMGCoA还原酶和LDLR的表达预防高脂饮食大鼠高血脂的发生.

The mechanism of bifidobacterium vinegar affecting the plasma lipid of rats fed with high-fat diet

Objective To discuss the prophylactic antiobesity effect of bifidobacterium vinegar and its possible mechanism. Methods 48 SD rats were randomly divided into 6 groups ： bifidobaeterium vinegar groups of three different doses, ordinary vineger group, high-fat model group and basic group. The possible mechanism of bifidobacterium vinegar affecting the plasma lipid of rats fed with high-fat diet was observed. The lipidoses in liver were measured; mRNA expression of LDLR and HMGCoA reductase in liver tissue were detected by immunohistochemistry and RT-PCR. Results The levels of HMGCoA reduetase of bifidobacterium vinegar groups were depressed obviously compared with that of high-fat model group. Conclusion Bifidobacterium vinegar can prevent the oecurance of high-fat in rats through adjusting the LDLR and HMGCoA reductase in liver to some degree.