Effect of different olive oil-derived antioxidants (hydroxytyrosol and 3,4-dihydroxyphenylglycol) on the quality of frozen-thawed ram sperm |
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| Institution: | 1. Department of Genetics, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, 14014, Spain;2. Instituto de la Grasa, Consejo Superior de Investigaciones Cientí?cas (CSIC), Sevilla, 41013, Spain;3. Centro Agropecuario Provincial de la Diputación de Cordoba, Cordoba, 14014, Spain;4. Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, 14014, Spain;1. Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran;2. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran;3. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;4. Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran;5. Department of Clinical and Experimental Medicine (IKE), Division of Clinical Sciences, Obstetrics and Gynecology, Linköping University, Linköping, Sweden;6. Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, Barcelona, Spain;1. Department of Genetics, University of Cordoba, 14014, Cordoba, Spain;2. Instituto de Investigación y Formación Agraria y Pesquera (IFAPA) Hinojosa Del Duque, Carretera el Viso, Km 2, 14270, Cordoba, Spain;3. Centro Agropecuario Provincial de la Diputación de Cordoba, 14014, Cordoba, Spain;4. Department of Animal Medicine and Surgery, University of Cordoba, 14014, Cordoba, Spain |
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| Abstract: | The aim of the present study was to evaluate the effect of the addition of different concentrations of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during the freezing-thawing process. Sperm was collected, pooled and diluted with commercial extenders and then divided into aliquots supplemented with different concentrations (10 μg/ml, 30 μg/ml, 50 μg/ml and 70 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. A control group, without antioxidant, was also prepared. Sperm motility, viability, acrosome integrity, mitochondrial membrane potential and lipid peroxidation (LPO) were assessed. The results showed that frozen-thawed ram spermatozoa exhibited lower values for motility, membrane integrity, acrosome and mitochondrial membrane potential than fresh samples (P ≤ 0.01). However, when antioxidants were added, thawed spermatozoa exhibited relatively low LPO, recording values similar to fresh spermatozoa; by contrast, the control group of frozen-thawed spermatozoa without antioxidants exhibited significantly higher LPO (P ≤ 0.01). The addition of a HT+DHPG mixture (MIX) had a negative impact on sperm membrane and acrosome integrity, suggesting that a pure antioxidant supplementation has the potential to offer superior results. In conclusion, HT and DHPG exhibited a positive effect on the frozen-thawed spermatozoa inasmuch as they reduced the LPO. These olive oil-derived antioxidants have the potential to improve frozen-thawed sperm quality, although further studies should be carried out to analyse the antioxidant effect at different times after thawing. |
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| Keywords: | Cryopreservation Olive oil Ovine Phenolic antioxidant Spermatozoa |
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