Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics |
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| Institution: | 1. Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran;2. Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Switzerland;1. Institute for Animal Health and Cattle Development, University of León, León, Spain;2. Department of Molecular Biology, University of León, León, Spain;3. Department of Medicine, Surgery and Veterinary Anatomy, University of León, León, Spain;4. Division for Research and Post-graduate Studies, Technological Institute of Conkal, Yucatán, México;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;3. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;4. INDEGSAL and Molecular Biology, University of León, León, Spain;5. Department of Animal Science, College of Agriculture, University of Kurdistan, Iran;1. College of Animal Science and Technology, Northwest A&F University, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Yangling, Shaanxi, 712100, PR China;2. Center of Northern Shaanxi White Cashmere Goats Engineering Research, Yulin University, Yulin, Shaanxi 719000, China;3. Center of Lhasa Animal Disease Prevention and Control, Lhasa, Tibet, 850000, China;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran;3. Animal Breeding Center of Iran, Karaj, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran |
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| Abstract: | This study was conducted to examine the effect of supplementation of Tris-egg yolk extender with lyophilized royal jelly (RJ) on chilled and frozen-thawed ram semen parameters. Ejaculates were collected by artificial vagina from 4 mature rams, twice a week for 4 weeks. Only samples with motility of ≥70% were included, pooled and divided into four equal parts and then diluted in extenders with various concentrations of RJ (0, 1, 3 and 5%, vol/vol) to a final concentration of 200 × 106 sperm/mL and was incubated at 37 °C for 30 min and were subsequently evaluated. After equilibration of extended semen for 2 h at 4 °C, some semen samples were packed in 0.25 mL plastic straws. Then, the straws were frozen in the liquid nitrogen vapor phase for 15 min and stored at ?196 °C in liquid nitrogen. The frozen straws were thawed in warm water (37 °C) for 30 s and evaluated; whereas, other semen samples were stored in the refrigerator (4 °C) up to 7 days. The chilled samples were kept in water bath (37 °C) for 5 min and then were evaluated. After dilution, the lowest and highest sperm total abnormality was recorded in 3 and 5% RJ supplemented groups, respectively (P < 0.05). The chilled sperm total motility and membrane integrity were significantly (P < 0.05) higher in 3% than those in 0% and 5% RJ supplemented groups. The chilled sperm progressive motility and viability was significantly (P < 0.05) higher in 1 and 3% than those in 0 and 5% RJ supplemented groups. The frozen-thawed sperm total motility, progressive motility, membrane integrity and viability were significantly higher in 3% RJ supplemented group (P < 0.05). In conclusion, supplementation of Tris-egg yolk extender with 3% lyophilized RJ had a protective effect on chilled and cryopreserved ram spermatozoa. |
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| Keywords: | Lyophilized royal jelly ram semen Extender Freezing-thawing Chilling |
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