Idebenone improves quality of ram sperm by mitigating oxidative stress during cryopreservation |
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| Institution: | 1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, 125001, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125004, India;1. School of Veterinary and Animal Science Medicine, Federal University of Bahia, Av. Adhemar de Barros, 500, Salvador, Bahia, 40170-110, Brazil;2. College of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, 18618-000, Brazil;3. Animal Reproduction Biotechnology Laboratory, Embrapa Coastal Tablelands, Av. Beira Mar 3250, Aracaju, Sergipe, 49025-040, Brazil;1. Animal Science Department, College of Agriculture, Isfahan University of Technology, P.O.Box: 84156/83111, Isfahan, Iran;2. Department of Reproductive Biotechnology, Reproductive Biotechnology Research Center, Avicenna Research Institute ACECR, Shahid Beheshti University, Tehran, Iran;3. Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Department of Animal Science, University of Illinois, Urbana, IL, USA |
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| Abstract: | The present study was designed to test the effect of different levels of idebenone, a potent antioxidant on the quality of ram semen at post thaw. Eighteen (18) ejaculates were collected and extended with tris extender supplemented with no antioxidant (CON), with 2 μM idebenone (Id2), 5 μM idebenone (Id5), 7.5 μM idebenone (Id7.5) and 10 μM idebenone (Id10). The sperm quality was determined in terms of percent sperm motility, live sperm percentage, percent hypoosmotic swelling test (HOST) positive spermatozoa and percent intact acrosome (PIA). Moreover, malondialdehyde (MDA) level, an end product of lipid peroxidation (LPO) was also measured at post thaw both in seminal plasma and sperm cell. At post thaw, the percent sperm motility was significantly higher (p < 0.05) for Id10 as compared to Id2, Id5, Id7.5 and control. The live sperm percentage was non-significantly (p > 0.05) higher for Id10 as compared to control, Id5 and Id7.5 but significantly higher than Id2. The percent HOST positive spermatozoa was significantly higher (p < 0.05) for Id10 than control, Id2 and Id5. The MDA level in seminal plasma was significantly lower (p < 0.05) for Id10 than control and Id2. The MDA level in spermatozoa did show similar trend as in seminal plasma. Further, all the sperm parameters at all idebenone levels declined significantly from pre freeze to post thaw. In conclusion, idebenone at 10 μM level improved post thaw sperm quality by mitigating peroxidative stress, hence could be considered as a promising antioxidant additive for cryopreservation of ram semen. |
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| Keywords: | Idebenone Oxidative stress Cryopreservation Semen Ram |
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