Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders |
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| Institution: | 1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa, 16059, Turkey;2. Beekeeping Development-Application and Research Center, Uludag University, Gorukle/Bursa, 16059, Turkey;3. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Nam?k Kemal University, Tekirdag, 59030, Turkey;1. Reproduction and Obstetrics Area, Animal Pathology Department, Universidad de Zaragoza, Spain;2. Infectious Diseases Area, Animal Pathology Department, Universidad de Zaragoza, Spain;1. UMR PRC, INRA 0085, CNRS 7247, Université de Tours, IFCE, F-37380, Nouzilly, France;2. UE 1255 APIS, INRA, F-17700, Surgères, France;1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;2. Department of Biostatistics, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;3. Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle/Bursa 16059, Turkey |
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| Abstract: | The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Sperm motility, plasma membrane functional integrity, and acrosomal integrity were evaluated. At post thaw, the highest sperm motility and acrosomal integrity rates were obtained in the RJ1 group. Functional integrity of sperm membrane was better preserved in the RJ1 and RJ2 groups compare to the other groups. The study shows that RJ supplemented extenders have beneficial effects on drone semen parameters. The results of the present study demonstrated advantage of using 1% RJ supplemented extender. |
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| Keywords: | Honey bee Apis mellifera Royal jelly Drone semen Cryopreservation |
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