Antibody recognition by a novel microgel photonic crystal |
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| Institution: | 1. Condensed Matter Physics Division, Materials Science Group, Indira Gandhi Centre for Atomic Research, HBNI, Kalpakkam, India;2. School of Physics, University of Hyderabad, Hyderabad, India;1. School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing 100083, PR China;2. State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China;3. Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing 100190, PR China;4. Beijing Key Laboratory of Ionic Liquids Clean Process, Chinese Academy of Sciences, Beijing 100190, PR China |
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| Abstract: | In this study, a easy-to-prepare biosensor for the sensitive detection of the antibody (Ab) protein was developed using a novel microgel photonic crystal (MPC). The MPC was fabricated by the spin-coated self-assembly method with the monodisperse Ab-sensitive poly (methyl methacrylate-acrylamide-glutaraldehyde-hapten) (P(MMA-AM-GA-HP)) microgels. Morphology characterization showed that the P(MMA-AM-GA-HP) microgels possessed round shapes and the large specific surface area, and the formed MPC had a highly ordered three dimensional (3D) periodically-ordered structure with the desired structural color. The Ab-response event of the P(MMA-AM-GA-HP) microgels can be directly transferred into a readable optical signal through a change in Bragg reflection of the periodic structure of the MPC. With the sensory system, the sensitive and selective detection of Ab was achieved without labeling techniques and expensive instruments. Therefore, this easy and sensitive detection system has great potential for next generation of the bioassay platform for clinical diagnosis and other applications. |
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| Keywords: | Biosensors P(MMA-AM-GA-HP) microgels Microgel photonic crystal Antibody protein detection |
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