Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1 |
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| Authors: | Graham K A Leithoff J Coe I R Mowles D Mackey J R Young J D Cass C E |
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| Institution: | Department of Oncology, University of Alberta, Cross Cancer Institute, Edmonton, Canada. |
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| Abstract: | The transportability of cytosine-containing nucleosides by recombinant hCNT1 was investigated in transfected mammalian cells. Apparent K(m) values for hCNT1-mediated transport of uridine, cytidine and deoxycytidine were, respectively, 59, 140 and 150 microM. Uridine transport was inhibited 89, 32 and 11%, respectively, by 500 microM gemcitabine, cytarabine and lamivudine, demonstrating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarabine and lamivudine are poor hCNT1 permeants. |
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