Biolistic transformation of soybean using a new selectable marker gene conferring resistance to dinitroanilines

A biolistic transformation of soybean using the construct pAHTUAm, which contains a mutant α-tubulin gene, as a selectable marker gene that confers resistance to dinitroaniline herbicides, as well as the additional construct pAHTUB1, which carries a full-length barley β-tubulin gene that ensures correct co-expression of exogenous tubulin in cells of transgenic soybean lines, is carried out. It is established that 10 μM trifluralin is the most optimal selective concentration to pick up transformed soybean lines. The transgenic nature of the selected regenerants is confirmed by Southern blotting hybridization using a specific probe to the α-tubulin selectable gene