水稻配子体,孢子体细胞质雄性不育系线粒体体外翻译产物的分析

对水稻配子体细胞质雄性不育系粤泰Ａ，保持系粤泰Ｂ，Ｆ１代泰优２号和孢子体细胞质雄性不育系珍汕９７Ａ，保持系珍汕９７Ｂ，Ｆ１代汕优６３及另一种孢子体细胞质雄性不育系马协Ａ，保持系马协Ｂ，Ｆ１代马协６３的黄化苗线粒体离体翻译产物进行ＳＤＳ－ＰＡＧＥ分析。结果表明：粤泰Ａ的线粒体比粤泰Ｂ，泰优２号少合成２条多肽，Ｂ与Ｆ１的带型相近，Ａ特异合成４０．７ｋＤ多肽；

Variation of Mitochondrial in vitro Translation Products of Gametophyte and Sporophyte Male Sterile Lines of Rice

Rice mitochondria were extracted and purified under sterile conditions fromyellow seedlings of gametophyte and sporophyte male sterile lines by centrifugation ina discontinuous sucrose gradient Mitochondrial proteins synthesized in vitro werelabelled by (35S) methionine. It showed that there were two polypeptides (52.0kD,65.4kD) synthesized in Yueha B (maintainer line) and Taiyou 2 (F, hybrid), butthey were suppressed in Yuetai A (sterile line). Mitochondria of Yuetri A synthesizeda characteristic polypeptide of 40.7kD which was suppressed in Yueni B and Taiyou2. Polypephdes differentially synthesized in WA type (A, B, F ) were not foundMaxie B (maintainer line) had also a characteristic polypeptide of 42.7kD. Apolypeptides of 67.8kD was synthesized only in Maxie B (maintainer line) and Maxie63 (F hybrid) and not detected in Maxie A (sterile line). These observations werediscussed with reference to the possible mechanisms of CMS in rice.