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嗜冷弧菌2693菌株的天冬氨酸转氨基甲酰酶基因克隆及测序
引用本文:张远富,徐莹.嗜冷弧菌2693菌株的天冬氨酸转氨基甲酰酶基因克隆及测序[J].遗传学报,1999,26(5):585-590.
作者姓名:张远富  徐莹
作者单位:中国预防医学科学院流行病学微生物学研究所!北京,102206,布鲁塞尔自由大学微生物研究所!比利时,布鲁塞尔自由大学微生物研究所!比利时,布鲁塞尔自由大学微生物研究所!比利时,布鲁塞尔自由大学微生物研究所!比利时
摘    要:对嗜冷弧菌2693菌株编码天冬氨酸氨甲酰基转移酶的基因进行了克隆,并对其核苷酸序列进行了分析。菌株2693的ATCase由pryBI操纵子pyrB编码催化链,pyrI编码调节莲。催化及调节多肽链由一条单一的双顺反操纵子编码,在同一启动基因控制之下进行转录。

关 键 词:嗜冷弧菌  天冬氨酸  转氨基甲酰酶  克隆  测序

Cloning and Sequence Analysis of ATCase Genes from Psychrophilic vibrio
ZHANG Yuan-Fu.Cloning and Sequence Analysis of ATCase Genes from Psychrophilic vibrio[J].Journal of Genetics and Genomics,1999,26(5):585-590.
Authors:ZHANG Yuan-Fu
Abstract:The gene encoding for aspartate transcarbamoylase (ATCase) from Psychrophilicvibrio, strain 2693 was cloned and sequenced. The sequence revealed the existence oftwo gene encoding respectively for a catalytic chain (pyrB) and a regulatory chain(pyr I). The catalytic and regulatory polypeptide chains of Vibrio 2693 ATCase areencoded by a single pyrBI bicistronic operon, and appear to be transcribed under thecontrol of the same promoter. The 3' -terminus of the catalytic cistron (pyrB) isadjacent to the. 5'-terminus of the regulatory cistron, these is only a 4 bp spacebetween the two coding regions.
Keywords:Psychrophilic vibrio  ATCas  pyrBI
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