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| 蝴蝶兰LFY基因克隆及高效植物表达载体的构建 | |
| 引用本文： | 崔波,牛苏燕,蒋素华,武思,王默菲,叶永忠. 蝴蝶兰LFY基因克隆及高效植物表达载体的构建[J]. 生物技术通报, 2012, 0(2): 65-68 |
| 作者姓名： | 崔波 牛苏燕 蒋素华 武思 王默菲 叶永忠 |
| 作者单位： | 1. 河南农业大学生命科学学院,郑州450002;郑州师范学院生物工程研究所,郑州450044 2. 河南农业大学生命科学学院,郑州,450002 3. 郑州师范学院生物工程研究所,郑州,450044 |
| 摘 要： | 根据NCBI中蝴蝶兰LFY花序分生组织基因序列设计2对引物,用RT-PCR法从蝴蝶兰花芽中扩增出LFY基因,对扩增产物进行克隆和测序.结果表明,获得的蝴蝶兰LFY基因约为1500 bp,与报道序列同源性达98.71％.将LFY基因插入pRI101-ON载体中,经PCR、双酶切及测序鉴定,证实重组表达质粒中含有目的片段,表明成功构建了高效植物表达载体pRI1O1-LFY. |
| 关 键 词： | 蝴蝶兰 LFY基因 克隆 高效植物表达载体构建 |
LFY Gene Cloning of Phalaenopsis and Construction of High-effective Plant Expression Vector |
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| Cui Bo , Niu Suyan , Jiang Suhua , Wu Si , Wang Mofei , Ye Yongzhong. LFY Gene Cloning of Phalaenopsis and Construction of High-effective Plant Expression Vector[J]. Biotechnology Bulletin, 2012, 0(2): 65-68 | |
| Authors: | Cui Bo Niu Suyan Jiang Suhua Wu Si Wang Mofei Ye Yongzhong |
| Affiliation: | 1(1College of Life Science,Henan Agricultural University,Zhengzhou 450002;2Institute of Bioengineering, Zhengzhou Normal College,Zhengzhou 450044) |
| Abstract: | Two pairs of primers were designed according to the reference Phalaenopsis LFY floral meristem identity gene from NCBI.The Phalaenopsis LFY floral meristem identity gene was amplified from the Phalaenopsis blossom bud by using RT-PCR.PCR product was cloned into the PMD19-T and sequenced.The sequencing result showed that the target gene was about 1 500 bp and the homology between the Phalaenopsis LFY floral meristem identity gene and the template was 98.71%.Inserting the Phalaenopsis LFY floral meristem identity gene into pRI101-ON vector.The recombinant plasmid pRI101-LFY was identified by PCR,double digests and sequencing.The result showed that the recombinant plasmid pRI101-LFY was constructed successfully. |
| Keywords: | Phalaenopsis LFY gene Cloning Construction of high-effective plant expression vector |
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