Protective Effect of Nitric Oxide against Oxidative Damage in <Emphasis Type="Italic">Arabidopsis</Emphasis> Leaves under Ultraviolet-B Irradiation |
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Authors: | Lingang Zhang Shuo Zhou Yi Xuan Miao Sun Liqun Zhao |
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Institution: | (1) Institute of Molecular Cell Biology, School of Life Sciences, Hebei Normal University, Shijiazhuang, 050016, China;(2) Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, 050016, China;(3) School of Life Sciences, Lanzhou University, Lanzhou, 730000, China;(4) Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China |
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Abstract: | Nitric oxide (NO) is a key molecule involved in many physiological processes. To characterize its roles in the tolerance of
Arabidopsis thaliana to ultraviolet-B (UV-B), we investigated the effect of a reduced endogenous NO level on oxidative damage to wild-type and
mutant (Atnoa1) plants. Under irradiation, hydrogen peroxide was accumulated more in mutant leaves than in the wild type. However, the amounts
of UV-B-absorbing compounds (flavonoids and anthocyanin) and the activities of two antioxidant enzymes—catalase (CAT, EC 1.11.1.6)
and ascorbate peroxidase (APX, EC 1.11.1.11)—were lower in leaves of the former. Supplementing with sodium nitroprusside,
an NO donor, could alleviate the oxidative damage to mutant leaves by increasing flavonoid and anthocyanin contents and enzyme
activities. In comparison, , an inhibitor of nitric oxide synthase, had the opposite effects on oxidation resistance in wild-type leaves. All these results
suggest that nitric oxide acts as a signal for an active oxygen-scavenging system that protects plants from oxidative stress
induced by UV-B irradiation. |
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Keywords: | Nitric oxide Oxidative damage Ultraviolet-B |
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