Two genetically distinct units of Sinomanglietia glauca (Magnoliaceae) detected by chloroplast PCR-SSCP |
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引用本文: | 张志荣,罗来春,吴丁,张志勇.Two genetically distinct units of Sinomanglietia glauca (Magnoliaceae) detected by chloroplast PCR-SSCP[J].植物分类学报,2009,47(2):110-114. |
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作者姓名: | 张志荣 罗来春 吴丁 张志勇 |
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作者单位: | 1. 江西农业大学亚热带生物多样性实验室;2. 景德镇高等专科学校; |
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摘 要: | Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP (single-stranded conformation polymorphism), including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units (ESUs) were recommended for effective conservation of S. glauca.
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关 键 词: | ISSR标记 PCR 叶绿体 华木莲 CP技术 单位 木兰科 单链构象多态性分析 |
收稿时间: | 2008-9-1 |
修稿时间: | 2008-12-5 |
Two genetically distinct units of Sinomanglietia glauca (Magnoliaceae) detected by chloroplast PCR-SSCP |
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Abstract: | chloroplast genome, genetic structure, PCR-SSCP, Sinomanglietia glauca. |
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Keywords: | chloroplast genome genetic structure PCR-SSCP Sinomanglietia glauca |
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