Short communication: Purification and properties of a glucose-forming amylase of Lactobacillus brevis |
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| Authors: | M O Ilori O O Amund O Omidiji |
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| Institution: | (1) Department of Botany and Microbiology, Lagos State University, P.M.B. 1087, Apapa, Lagos, Nigeria;(2) Department of Biological Sciences, University of Lagos, Akoka, Lagos, Nigeria |
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| Abstract: | An extracellular glucose-forming amylase was produced by Lactobacillus brevis isolated from  Kagasok tea . The enzyme was purified 70-fold and had optimal activity at 55°C and pH 6.5. Its K
m value for starch was 0.27 mg ml-1 and its M
r was approx. 75,900 Da. The activity of the enzyme was enhanced by Ca2+, Mg2+, Na+ or K+ and inhibited by EDTA, KCN, citric acid and l-cysteine. |
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| Keywords: | Amylase inhibitors Lactobacillus purification |
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