Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa

The results of establishing an in vitro culture, plantlet regeneration, and rooting of Camelina sativa cultivar Peremozhets and cultivar Mirazh are presented. The effective concentrations of sterilizing agents and the duration of plant material treatment were estimated. The phytohormone ratio, the sucrose concentration in the nutrient medium that induced the effective formation of C. sativa shoots, and the NAA concentration for plantlet rooting have been established. A method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying the reporter β-glucoronidase (gus) gene driven under the 35S CaMV promoter and nos-terminator, as well as the selective marker hpt gene conferring hygromycin-resistance in transgenic plant, was elaborated.