Efficient Protein Expression in <Emphasis Type="Italic">Bombyx mori</Emphasis> Larvae of the Strain d17 Highly Sensitive to <Emphasis Type="Italic">B. mori</Emphasis> Nucleopolyhedrovirus |
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| Authors: | Naoya Kawakami Jae Man Lee Hiroaki Mon Yuji Kubo Yutaka Banno Yutaka Kawaguchi Katsumi Maenaka Enoch Y Park Katsumi Koga Takahiro Kusakabe |
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| Institution: | (1) Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku Fukuoka 812–8581, Japan;(2) Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University Graduate School, Fukuoka 812–8581, Japan;(3) Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University Graduate School, 3-1-1 Maidashi, Higashi-ku Fukuoka 812–8582, Japan;(4) Department of Applied Biological Chemistry Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422–8529, Japan;(5) Department of Biological Substances and Life Science, Kyushu Kyoritsu University, 1–8 Jiyugaoka, Yahata-Nishi-ku Kitakyushu 807–8585, Japan |
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| Abstract: | The recombinant protein expression by Bombyx mori nucleopolyhedrovirus (BmNPV) infecting silkworm larvae or pupae may endow us with a potent system for the production of large
eukaryotic proteins. However, the screening of silkworm strains ideally suited to this method has scarcely been conducted.
In the present study, we injected recombinant BmNPV containing a reporter gene, luciferase or DsRed, into hemocoel of fifth
instar larvae of selected 12 silkworm strains. Among them, the strain d17 is found to be the highest in reporter expression
from the intrinsic polyhedrin promoter of Autographa californica NPV or the silkworm actin A3 promoter. These results suggest that the d17 strain is highly permissive for BmNPV replication
and is the most likely candidate of a “factory” for large-scale expression using the BmNPV bacmid system. |
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| Keywords: | BmNPV Silkworm strains Large-scale expression Luciferase DsRed |
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