Cloning,characterization and localization of <Emphasis Type="Italic">CHS</Emphasis> gene from blood orange, <Emphasis Type="Italic">Citrus sinensis</Emphasis> (L.) Osbeck cv. Ruby |
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Authors: | Xu Lu Wei Zhou Feng Gao |
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Institution: | (1) School of Life Sciences, South China Normal University, 510631 Guangzhou, People’s Republic of China |
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Abstract: | Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named
as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids.
Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other
plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the
protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that
at least three copies of CsCHS-bo were present in the genome.
The novel nucleotide sequence data published here have been deposited in the EMBL/DDBJ/GenBank databases under accession number
EU410483. |
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Keywords: | Chalcone synthase gene Citrus sinensis (L ) Osbeck cv Ruby Anthocyanins biosynthesis Fluorescence in situ hybridization (FISH) |
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