Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresis. |
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| Authors: | E H McConkey |
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| Institution: | Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 USA |
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| Abstract: | Comparison of complex protein mixtures by high-resolution, two-dimensional polyacrylamide gel electrophoresis is simplified by coelectrophoresis of 3H-labeled and 14C-labeled preparations, followed by two-step autoradiography. Both qualitative and quantitative comparisons are possible. Applications to genetics, molecular evolution, and metabolism are described. |
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