Determination of bergenin based on the electrochemiluminescence quenching of tris(2,2′‐bipyridine)‐ruthenium(II) |
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Authors: | Yinggui Zhu Lili Zou Qin Dong Derong Jiang |
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Institution: | College of Chemistry and Material Science, Anhui Normal University, Wuhu, China |
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Abstract: | Quenching effects of bergenin, based on the electrochemiluminescence (ECL) of the tris(2,2′‐bipyridyl)‐ruthenium(II) (Ru(bpy)32+)/tri‐n‐propylamine (TPrA) system in aqueous solution, is been described. The quenching behavior can be observed with a 100‐fold excess of bergenin over Ru(bpy)32+. In the presence of 0.1 m TPrA, the Stern–Volmer constant (KSV) of the ECL quenching is as high as 1.16 × 104 M?1 for bergenin. The logarithmic plot of the inhibited ECL versus logarithmic plot of the concentration of bergenin was linear over the range 3.0 × 10?6–1.0 × 10?4 mol/L. The corresponding limit of detection was 6.0 × 10?7 mol/L for bergenin (S/N = 3). In the mechanism of quenching it is believed that the competition of the active free radicals between Ru(bpy)32+/TPrA and bergenin was the key factor for the ECL inhibition of the system. Photoluminescence, cyclic voltammetry, coupled with bulk electrolysis, supports the supposition mechanism of the Ru(bpy)32+/TPrA–bergenin system. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | bergenin electrochemiluminescence (ECL) quenching |
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