Employment of 4‐(1,2,4‐triazol‐1‐yl)phenol as a signal enhancer of the chemiluminescent luminol–H2O2–horseradish peroxidase reaction for detection of hepatitis C virus in real samples |
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| Authors: | Jian Liu Lili Zhang Chuanyun Fu Yunshan Wang Shanhui Sun |
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| Institution: | 1. School of Control Science and Engineering, Shandong University, Jinan, China;2. School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan, China;3. Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China;4. Jinan Central Hospital Affiliated to Shandong University, Jinan, China |
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| Abstract: | Highly sensitive detection of hepatitis C virus (HCV) in serum is a key method for diagnosing and classifying the extent of HCV infection. In this study, a p‐phenol derivative, 4‐(1,2,4‐triazol‐1‐yl)phenol (4‐TRP), was employed as an efficient enhancer of the luminol–hydrogen peroxide (H2O2)–horseradish peroxidase (HRP) chemiluminescence (CL) system for detection of HCV. Compared with a traditional enhancer, 4‐TRP strongly enhanced CL intensity with the effect of prolonging and stabilizing light emission. The developed CL system was applied to detecting HCV core antigen (HCV‐cAg) using a sandwich structure inside microwells. Our experimental results showed that there was good linear relationship between CL intensity and HCV‐cAg concentration in the 0.6–3.6 pg/mL range (R = 0.99). The intra‐ and inter‐assay coefficients of variation were 4.5–5.8% and 5.0–7.3%, respectively. In addition, sensitive determination of HCV‐cAg in serum samples using the luminol–H2O2–HRP–4‐TRP CL system was also feasible in clinical settings. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | Chemiluminescence Signal enhancer Hepatitis C virus Luminol |
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