Co-culture of human gastric endoscopic biopsies with Helicobacter pylori: a simple method for studying early phases of bacteria-host interaction |
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| Authors: | Pellicanò Antonella Leone Isabella Imeneo Maria Amorosi Andrea Luzza Francesco |
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| Institution: | Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro "Magna Graecia", Campus Universitario di Germaneto, Viale Europa 88100 Catanzaro, Italy. |
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| Abstract: | Gastric biopsies obtained through endoscopy from patients uninfected by Helicobacter pylori were co-cultured with an H. pylori strain. According to tissue and H. pylori viability, interleukin 8 was increased in biopsy homogenate and supernatant after 12-36h culture. This simple method is suitable to investigate early phases of bacteria-host interaction. |
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