首页 | 本学科首页   官方微博 | 高级检索  
     


Interaction of exogenous benzoquinone with Photosystem II in chloroplasts. The semiquinone form acts as a dichlorophenyldimethylurea-insensitive secondary acceptor
Authors:Jérôme Lavergne
Affiliation:Laboratoire de Photosynthèse, C.N.R.S., 91190 Gif-sur-Yvette France
Abstract:Chloroplasts were submitted to a sequence of saturating short flashes and then rapidly mixed with dichlorophenyldimethylurea (DCMU). The amount of singly reduced secondary acceptor (B?) present was estimated from the DCMU-induced increase in fluorescence in the dark caused by the reaction: QB?
/></figure> Q<sup>?B</sup>. By varying the time interval between the preillumination and the mixing, the time course of B<sup>?</sup> reoxidation by externally added benzoquinone was investigated. It was found that benzoquinone oxidizes B<sup>?</sup> in a bimolecular reaction, and does not interact directly with Q<sup>?</sup>. When a sufficient delay after the preillumination was allowed in order to let benzoquinone reoxidize B<sup>?</sup> before the injection of DCMU, the fluorescence increase caused by one subsequent flash fired in the presence of DCMU was followed by a fast decay phase (<math><mtext>t</mtext><msub><mi></mi><mn><mtext>1</mtext><mtext>2</mtext></mn></msub><mtext> ? 100 μ</mtext><mtext>s</mtext></math>). The amplitude of this phase was proportional to the amount of B<sup>?</sup> produced by the preillumination. This fast decay was observed only after the first flash in the presence of DCMU. These results are interpreted by assuming a binding of the singly reduced benzoquinone to Photosystem II where it acts as an efficient, DCMU-insensitive, secondary (exogenous) acceptor.</td>
		  </tr> 
		  <tr>
		   <td align=
Keywords:Photosystem II  Benzoquinone  Chlorophyll fluorescence  Dichlorophenyldimethylurea  Electron acceptor  (Spinach chloroplast)  DBMIB  dibromothymoquinone  DCMU  3-(3,4-dichlorophenyl)-1,1-dimethylurea  PS  photosystem
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号