Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs |
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| Authors: | Z M Banjar L S Hnilica R C Briggs J Stein G Stein |
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| Institution: | 1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 U.S.A.;2. Department of Biochemistry and Molecular Biology, University of Florida School of Medicine, Gainesville, FL 32610 U.S.A. |
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| Abstract: | Immunochemical techniques were used to investigate the protein-DNA crosslinking by ultraviolet (UV) and gamma radiation as well as 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) or cis- and trans-diamminedichloroplatinum II (cis-DDP and trans-DDP). Antisera to 0.35 M NaCl extract and 0.35 M NaCl residue of HeLa nuclei were employed. Both gamma and UV irradiation, exposure to cis- or trans-DDP and, to a lesser extent, BCNU, resulted in crosslinking of various antigens to the DNA. Although several antigens were crosslinked by all the employed agents, other exhibited agent-specific crosslinking patterns. |
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| Keywords: | BCNU 1 3-bis-(2-chloroethyl)-1-nitrosourea cis-DDP cis-diamminedichloroplatinum trans-DDP trans-diamminedichloroplatinum PMSF phenyl-methylsulfonylfluoride UV ultra violet SDS sodium dodecyl sulfate PAP peroxidaseantiperoxidase |
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