Localization of alkaline phosphatase inBacillus intermedius cells |
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Authors: | M P Sharipova N P Balaban I B Leshchinskaya |
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Institution: | (1) Kazan State University, ul. Kremlevskaya 18, 420008 Kazan, Russia |
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Abstract: | Alkaline phosphatase, an enzyme secreted byBacillus intermedius S3-19 cells to the medium, was also detected in the cell wall, membrane, and cytoplasm. The relative content of alkaline
phosphatase in these cell compartments depended on the culture age and cultivation medium. The vegetative growth ofB. intermedius on 0.3% lactate was characterized by increased activity of extracellular and membrane-bound phosphatases. The increase in
lactate concentration to 3% did not affect the activity of membrane-bound phosphatase but led to a decrease in the activity
of the extracellular enzyme. Na2HPO4 at a concentration of 0.01 % diminished the activity of membrane-bound and extracellular phosphatases. CoCl2 at a concentration of 0.1 mM released membrane-bound phosphatase into the medium. By the onset of sporulation, phosphatase
was predominantly localized in the medium and in the cell wall. As is evident from zymograms, the multiple molecular forms
of phosphatase varied depending on its cellular localization and growth phase. |
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Keywords: | alkaline phosphatase ofBacillus intermedius membrane-bound form localization |
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