Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils |
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| Authors: | Pedro A.G. Santos A. Cristina Figueiredo Pedro M.L. Lourenço José G. Barroso Luis G. Pedro M. Margarida Oliveira Jan Schripsema Stanley G. Deans Johannes J.C. Scheffer |
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| Affiliation: | (1) Centro de Biotecnologia Vegetal, Faculdade de Cîencias de Lisboa, Departamento de Biologia Vegetal, Bloco C2, Campo Grande, 1749-016 Lisbon, Portugal;(2) ITQB/IBET, Quinta do Marqûes, Aptd. 12, 2781-901 Oeiras, Portugal;(3) Setor de Quimica de Produtos Naturais, LCQUI/CCT, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28015-620 Campo dos Goytacazes, Rio de Janeiro, Brasil;(4) Department of Food Science & Technology, SAC, Auchincruive, Ayr, KA6 5HW, Scotland, UK;(5) Division of Pharmacognosy, LACDR, Leiden University, Gorlaeus Laboratories, PO Box 9502, 2300 RA Leiden, The Netherlands |
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| Abstract: | Transformed root cultures of Anethum graveolens were induced by inoculation of aseptically grown seedlings with Agrobacterium rhizogenes carrying plasmid pRi 1855. The main component of the essential oils from the fruits and from the roots of the parent plant was carvone, whereas  -phellandrene and apiole were dominant in the oil from, respectively, the aerial parts and the hairy roots. The essential oils from the fruits, aerial parts and roots of the parent plant were at 2%, 0.3% and 0.06% (v/w), respectively, but only 0.02% (v/w) in the hairy root cultures. Growth of the hairy root cultures reached 600 mg dry wt/50 ml medium after 50 days. The essential oil composition did not change significantly during their growth. |
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| Keywords: | Agrobacterium rhizogenes Anethum graveolens Apiaceae essential oil hairy root cultures |
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