Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2-13.1, and two pseudogenes on chromosomes 1q23-24 and 22q11.2-13.1 |
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| Authors: | N. Kunze G. C. Yang Z. Y. Jiang H. Hameister S. Adolph K. -H. Wiedorn A. Richter R. Knippers |
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| Affiliation: | (1) Fakultät für Biologic der Universität, Postfach 5560, D-7750 Konstanz, Germany;(2) Abteilung Klinische Genetik der Universität, Oberer Eselsberg, M 25, D-7900 Ulm, Germany;(3) Institut für Humangenetik und Genetische Poliklinik der Universität, Bahnhofstrasse 7a, D-3550 Marburg, Germany |
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| Abstract: | Summary Different subfragments of a cDNA coding for DNA topoisomerase I were used as probes to determine the chromosomal localization of topoisomerase I sequences in human cells. Southern blotting of restricted DNA from a panel of rodent-human somatic cell hybrids revealed the localization of the complete gene on chromosome 20 and the presence of two truncated topoisomerase I pseudogene sequences on chromosomes 1 and 22. In situ chromosome hybridzation experiments confirmed these results showing the location of the complete gene on band q11.2–13.1 of chromosome 20, and the location of the pseudogene sequences on band q23–24 of chromosome 1 and q11.2–13.1 of chromosome 22. |
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