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Conformational Analysis of the Phosphorylated Epidermal Growth Factor Receptor |
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| Authors: | Anupam Bishayee Laura Beguinot Subal Bishayee |
| Institution: | (1) Department of Pathology and Laboratory Medicine, UMDNJ-New Jersey Medical School, MSB C-567, 185 S. Orange Avenue, Newark, NJ-, 07103-2714, U.S.A;(2) Molecular Oncology Unit, DIBIT and CNR Institute of Neuroscience and BioImaging, H. S. Raffaele Via Olgettina 58, 20132 Milano, Italy |
| Abstract: | Phosphorylation-induced conformational changes have been well documented with different receptor tyrosine kinases. However, the susceptible epitopes and the tyrosine residue(s) involved in particular structural alteration mostly remain to be determined. Using a conformation-specific anti-peptide antibody, we have not only identified one such domain in the C-terminal tail of the EGF receptor but also identified the phosphate acceptor sites that are involved in the conformational change. |
| Keywords: | Antigenic determinant Autophosphorylation sites Conformation-specific antibody EGF receptor Receptor tyrosine kinase |
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