rRNA topography in ribosome. IV. The accessibility of the 5'-end region of 16S rRNA |
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| Authors: | E A Skripkin A M Kopylov A A Bogdanov S V Vinogradov Yu A Berlin |
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| Institution: | (1) A.N. Belozersky Laboratory of Bioorganic Chemistry and Molecular Biology, Moscow State University, 117234 Moscow, USSR;(2) M.M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, 117312 Moscow, USSR |
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| Abstract: | The accessibility of the 5'-end region of 16S rRNA (A8GAGUUUG15) inEscherichia coli ribosomes for complementary binding with the synthetic octanucleotide d(CAAACTCT) has been studied. Nonequilibrium gel-filtration was used to evaluate parameters of the binding of this oligonucleotide with free 16S rRNA, ribosomal subunits, and 70S ribosomes. A simple approach is presented to calculate the apparent association constants and the number of binding sites based upon the data obtained under those conditions. Free 16S rRNA, 30S subunits, and 70S ribosomes were found to form rather stable complexes with the octanucleotide, the association constants being similar in all three cases. These data strongly suggest the surface location of the 16S rRNA 5'-end inE. coli ribosomes. |
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