Regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells in PEG-ECM hydrogels |
| |
Authors: | Nathaniel S Hwang Shyni Varghese Hanwei Li Jennifer Elisseeff |
| |
Institution: | (1) Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins School of Medicine, 400 N Broadway, Smith 5035 Baltimore, MD, USA;(2) Department of Bioengineering, University of California at San Diego, La Jolla, CA 92092, USA;(3) David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; |
| |
Abstract: | Bone-marrow-derived mesenchymal stem cells (MSCs) are candidates for regeneration applications in musculoskeletal tissue such
as cartilage and bone. Various soluble factors in the form of growth factors and cytokines have been widely studied for directing
the chondrogenic and osteogenic differentiation of MSCs, but little is known about the way that the composition of extracellular
matrix (ECM) components in three-dimensional microenvironments plays a role in regulating the differentiation of MSCs. To
define whether ECM components influence the regulation of osteogenic and chondrogenic differentiation by MSCs, we encapsulated
MSCs in poly-(ethylene glycol)-based (PEG-based) hydrogels containing exogenous type I collagen, type II collagen, or hyaluronic
acids (HA) and cultured them for up to 6 weeks in chondrogenic medium containing transforming growth factor-β1 (10 ng/ml)
or osteogenic medium. Actin cytoskeleton organization and cellular morphology were strongly dependent on which ECM components
were added to the PEG-based hydrogels. Additionally, chondrogenic differentiation of MSCs was marginally enhanced in collagen-matrix-based
hydrogels, whereas osteogenic differentiation, as measured by calcium accumulation, was induced in HA-containing hydrogels.
Thus, the microenvironments created by exogenous ECM components seem to modulate the fate of MSC differentiation. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|