Assessment of genetic fidelity of micropropagated plants of <Emphasis Type="Italic">Simmondsia chinensis</Emphasis> (Link) Schneider using RAPD and ISSR markers |
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Authors: | Sunil Kumar Manisha Mangal A K Dhawan Narender Singh |
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Institution: | (1) Centre for Plant Biotechnology, CCS Haryana Agricultural University New Campus, Hisar, 125 004, India;(2) Department of Botany, Kurukshetra University, Kurukshetra, 136 119, India; |
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Abstract: | RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) markers were screened to test the genetic
integrity of jojoba (Simmondsia chinensis) plants multiplied through axillary bud multiplication from nodal segments. The in vitro raised plantlets were maintained
for up to 12 in vitro subcultures. During the study a total of 48 (32 RAPD and 16 ISSR) primers were screened, out of which
24 RAPD and 13 ISSR primers produced a total of 191 (126 RAPD and 65 ISSR) clear, distinct and reproducible amplicons. The
amplified products were monomorphic across all the selected micropropagated plants and were similar to the mother plant. The
micropropagation protocol developed by our group for rapid in vitro multiplication is appropriate for clonal propagation of
jojoba. The outcome supports the fact that axillary bud multiplication can also be used as one of the safest modes for the
production of true-to-type plants. |
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