Interactions of acetylcholine receptor and acetylcholinesterase with lipid monolayers |
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| Authors: | Therese Wiedmer Urs Brodbeck Peter Zahler Bernard W Fulpius |
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| Institution: | 1. Medizinisch-chemisches Institut der Universität, Bühlstrasse 28, 3000 Bern 9, Switzerland;2. Biochemisches Institut, Freiestr. 3, 3000 Bern 9, Switzerland;3. Département de Biochimie, Sciences II, 1211 Genève 4, Switzerland |
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| Abstract: | The interaction of acetylcholine receptor and acetylcholinesterase with lipid monolayers was followed by measuring changes in surface pressure.When injected into the subphase of a lipid monolayer, the proteins caused increases in surface pressure from 5 to 10 dynes/cm, indicating a penetration of protein into the monolayer. At pH values below the isoelectric point of the proteins the incorporation was improved. The same was observed when Ca2+ (2 mM) was added.The presence of the enzyme in the mixed film could be demonstrated by using diiso3H]propyl fluorophosphate-labelled acetylcholinesterase as well as by measuring enzyme activity. Acetylcholine receptor was shown to be present in the mixed film by using a complex made of the receptor and α-3H]neurotoxin. |
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| Keywords: | BBOT diisopropyl fluorophosphate 5 5′-dithiobis(2-nitro-benzoic acid) To whom correspondence should be addressed |
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