Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of <Emphasis Type="Italic">eruca sativa</Emphasis> mill |
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| Authors: | T?Zhang Z?Y?Cao Email author" target="_blank">X?Y?WangEmail author |
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| Institution: | (1) The Institute of Cell Biology, Lanzhou University, 730000 Lanzhou, P. R. China;(2) The Institute of Industrial Crops, Gansu Academy of Agricultural Science, 730070 Lanzhou, P.R. China;(3) School of Life Science and Technology, Gansu Agricultural University, 730070 Lanzhou, P. R. China |
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| Abstract: | Summary A protocol was developed for high frequency somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants
of Eruca sativa. Explants grown on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-D formed embryogenic callus after 4 wk of culture. Secondary somatic embryos were also produced from primary somatic
embryos on MS medium containing 0.56 μM 2,4-D. Somatic embryos developed into mature embryos on MS medium in the presence of 45 gl−1 polyethylene glycol. After desiccation, somatic embryos developed into plantlets by culturing the mature somatic embryos
on 1/2 x MS medium containing 0.24 μM indole-3-butyric acid. |
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| Keywords: | embryogenic callus induction plant growth regulators plantlets somatic embryos |
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