体外遗传学方法及应用

体外遗传学是利用核酸分子本身一定的表型（如结合、催化等）在试管中分离筛选特定核酸分子序列进行研究的方法．由于体外遗传学方法改变了自然界缓慢的进化过程，使人为的进化得以简单地实现，同时也使许多核酸功能区的识别和确定由被动变为主动，为进一步探索基因的调控规律及主动地调节生化反应过程提供了有效的手段，近年来体外遗传学方法及在分子生物学方面的应用得到了很大的发展．

The Methods and Applications of In Vitro Genetics

In vitro genetics is a method for isolating and studying functional nucleic acid molecules in test-tubes based on their special phenotypes (e. g. binding properties, catalytic properties). In vitro genetic provides an effective, active method for determinating and artificial evolution of nucleic acid function sequences,so it was widely used in investigating the regulation of gene expression and biochemical reaction.The progress of in vitro genetic methods and applications in recent years is reviewed.