| 快速检查寡聚DNA片段序列的方法 |
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| 引用本文： | 朱榴琴,吴宇杰.快速检查寡聚DNA片段序列的方法[J].生物化学与生物物理进展,1996,23(3):284-285. |
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| 作者姓名： | 朱榴琴 吴宇杰 |
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| 作者单位： | 中国科学院生物物理研究所;中国科学院生物物理研究所 |
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| 摘 要： | 以质粒为模板，用待测寡聚DNA片段和通用测序引物进行PCR（聚合酶链式反应），PCR片段经纯化后插入到pUC－18或pUC－19的多克隆位点中，然后用通用测序引物测定重组质粒上待测寡聚DNA片段，即可清晰、正确地知道它的序列．
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| 关 键 词： | PCR 寡核苷酸 DNA序列 |
| 收稿时间： | 1995/8/14 0:00:00 |
| 修稿时间： | 1995/9/25 0:00:00 |
A Method to Confirm the Sequence of a Defined DNA Primer |
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| Zhu Liuqin and Wu Yujie.A Method to Confirm the Sequence of a Defined DNA Primer[J].Progress In Biochemistry and Biophysics,1996,23(3):284-285. |
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| Authors: | Zhu Liuqin and Wu Yujie |
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| Institution: | Institute of Biophysics, Academia Sinica;Institute of Biophysics, Academia Sinica |
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| Abstract: | The sequence of a defined DNA primer can be detected easily by a method described as below. A PCR (polymerase chain reaction) was carried out with the primer to be detected and one of the M13 universal sequencing primers. The resulting PCR fragment was purified and inserted into a pUC-18 or pUC-19 at the multicloning sites. Then the sequence of the primer to be detected can be exactly known by sequencing the recombinated plasmid with a M13 universal sequencing primer. |
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| Keywords: | PCR oligonucleotide DNA sequence |
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