| 随机引物在分子生物学研究中的应用 |
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| 引用本文： | 刘春宇,张春玲,夏家辉.随机引物在分子生物学研究中的应用[J].生物化学与生物物理进展,1996,23(6):517-520. |
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| 作者姓名： | 刘春宇 张春玲 夏家辉 |
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| 作者单位： | 湖南医科大学医学遗传学国家重点实验室;湖南农业大学生物技术系;湖南医科大学医学遗传学国家重点实验室 |
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| 摘 要： | 随机引物指非特异序列的寡聚核苷酸作为DNA合成过程中的引物, 是相对于特异引物的概念.90年代它与PCR技术结合衍生了几项新技术:采用不同长度随机引物进行DNA指纹分析而衍生出的RAPD、AP-PCR及DAF方法; 进行mRNA多态分析的“差异显示”; 以及rPCR, T-PCR等技术. 以RAPD为例介绍了随机引物PCR的技术特点及其在分子生物学研究中的应用.
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| 关 键 词： | 随机引物 任意引物 PCR 分子生物学 |
| 收稿时间： | 1995/12/14 0:00:00 |
| 修稿时间： | 1996/4/22 0:00:00 |
Application of Random Primers in the Research of Molecular Biology |
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| Liu Chunyu,Zhang Chuming and Xia Jiahui.Application of Random Primers in the Research of Molecular Biology[J].Progress In Biochemistry and Biophysics,1996,23(6):517-520. |
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| Authors: | Liu Chunyu Zhang Chuming and Xia Jiahui |
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| Institution: | Hunan Medical University, National Laboratory of Medical Genetics;Hunan Agriculture University, Deportment of Biotechnology;Hunan Medical University, National Laboratory of Medical Genetics |
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| Abstract: | Random or arbitrary primers, in contrast with common specific primer,refer to the non-specific oligonucleotides used as primers in DNA synthesis.In 1990's several new techniques were developed as a result of combination of random primers and PCR. RAPD, AP-PCR and DAF by random primers with different length were used for DNA fingerprinting. Differential display was used for polymorphism analysis of mRNA.rPCR,T-PCR and so on derived from application of random primers were discussed too.The technique characters and applications in molecular biology research of random primered PCR were introduced particularly in RAPD. |
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| Keywords: | random primer arbitrary primer PCR molecular biology |
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