| 锌7与镉7－金属硫蛋白清除羟自由基的比较 |
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| 引用本文： | 岳新萍,周杰昊,程时. 锌7与镉7－金属硫蛋白清除羟自由基的比较[J]. 生物化学与生物物理进展, 1996, 23(4): 352-355 |
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| 作者姓名： | 岳新萍 周杰昊 程时 |
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| 作者单位： | 北京医科大学生物物理系;北京医科大学生物物理系;北京医科大学生物物理系 |
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| 基金项目： | 国家自然科学基金资助29270356. |
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| 摘 要： | 分离及纯化兔肝金属硫蛋白.制备去金属金属硫蛋白、锌7与镉7金属硫蛋白．在不同pH条件下，比较后二者清除羟自由基能力；在pH6条件下，比较锌7－金属硫蛋白与有关蛋白和无机锌盐清除羟自由基效果．结论是在近生理pH条件下锌7－金属硫蛋白清除羟自由基能力远强于镉7－金属硫蛋白．金属硫蛋白清除羟自由基的能力主要来源于蛋白中处于还原态的流基．
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| 关 键 词： | 金属硫蛋白 锌7－金属硫蛋白 ESR顺磁捕集 羟自由基 还原态流基 |
| 收稿时间： | 1995-10-19 |
| 修稿时间： | 1996-02-11 |
A Comparison of Scavenging Hydroxyl Radical Between Zn7- and Cd7-Metallothionein |
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| Yue Xinping,Zhou Jiehao and Cheng Shi. A Comparison of Scavenging Hydroxyl Radical Between Zn7- and Cd7-Metallothionein[J]. Progress In Biochemistry and Biophysics, 1996, 23(4): 352-355 |
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| Authors: | Yue Xinping Zhou Jiehao Cheng Shi |
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| Affiliation: | Department of Biophysics, Beijing Medical Uniuersity;Department of Biophysics, Beijing Medical Uniuersity;Department of Biophysics, Beijing Medical Uniuersity |
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| Abstract: | Metallothionein (rabbit liver)(MT),apo-MT, Zn7-MT and Cd7-MT were prepared respectively. The scavenging effects of Zn7-MT and Cd7-MT on hydroxyl radical were compared at various pH values, and at pH 6 the effects of Zn7-MT were compared with related proteins and inorganic zinc salt. From these results it is concluded that the ability of Zn7-MT to scavenge hydroxyl radical is far stronger than that of Cd7MT and that the ability of MT to scavenge hydroxyl radical derives from reduced -SH group in MT. |
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| Keywords: | metallothionein hydroxyl radical Zn-metallothionein ESR spin trapping reduced-SH group |
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