首页 | 本学科首页   官方微博 | 高级检索  
     


Embryogenesis and genetic stability in long term megagametophyte-derived cultures of larch
Authors:von Aderkas  P.  Pattanavibool  R.  Hristoforoglu  K.  Ma  Y.
Affiliation:(1) Graduate Centre for Forest Biology, Department of Biology, University of Victoria, Victoria, BC, V8W 3N5, Canada;(2) Royal Forestry Department, Bangkok, Thailand;(3) Austrian Research Centers Seibersdorf, A-2444 Seibersdorf, Austria;(4) Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada
Abstract:Embryogenic cultures derived from megagametophytes of Larix decidua were maintained for up to 17 years. A few lines were divided into sub-lines, which were maintained in the same manner as the others. Embryogenic tissue was grown on 1/2 strength LM medium supplemented with glutamine and casein hydrolysate at constant temperature and light regimes. Chromosome counts were conducted at various times. DNA content was assessed by flow cytometry. Embryogenesis was monitored with each transfer and records of all appearances of green mature embryos were kept. Chromosome number was found to vary. DNA content and chromosome number, both of which had doubled a number of years after initiation, stabilized around 24 chromosomes for most cultures. A few lines showed substantial increases in chromosome number. One of these lines lost vigour and died. Another line showed a further doubling of DNA content. No lines were embryogenic over the entire period. Embryogenicity was lost completely in some lines, but in others the loss was temporary, as periodic restoration of embryogenesis was observed.
Keywords:chromosomes  flow cytometry  gynogenesis  haploid  Larix decidua  somaclonal variation
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号