Heterologous transformation of Cochliobolus lunatus |
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| Authors: | Marina Dermastia Damjana Rozman Radovan Komel |
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| Affiliation: | Institute of Biochemistry, Medical Faculty, University of Ljubljana, Yugoslavia. |
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| Abstract: | A DNA mediated transformation system has been developed for the filamentous fungus Cochliobolus lunatus. Transformants were obtained by using plasmid pAN 7-1 carrying the Escherichia coli hygromycin B phosphotransferase gene (hph) fused to an Aspergillus nidulans promoter. The integration of plasmid pAN 7-1 into the fungal genome altered the ability of this microorganism to transform progesterone. |
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| Keywords: | Cochliobolus lunatus Plasmid pAN 7–1 Genetic transformation Steroid bioconversion Progesterone |
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