Distribution of different forms of sterols in three cellular subfractions of Calendula officinalis leaves |
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| Authors: | Wirginia Janiszowska Ewa Sobocińska Zofia Kasprzyk |
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| Institution: | Institute of Biochemistry, University of Warsaw, Al. Z?wirki i Wigury 93, Poland |
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| Abstract: | From Calendula officinalis leaves three cellular subtractions (mitochondrial, Golgi membranes and microsomal) were obtained and enzymatically characterized. The contents of Δ0, Δ5, Δ7, Δ5, 22 sterols, as well as those of 24-methylenecholesterol and clerosterol, in the free and bound in the form of esters, glucosides and acylated glucosides were determined in these fractions. The results revealed the predominance of free sterols in the microsomal fraction, of esters in the mitochondrial fraction and of steryl glucosides and acylated glucosides in the Golgi fraction. |
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| Keywords: | Compositae intracellular distribution sterols 24-methylene-cholesterol clerosterol free and bound steryl forms |
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